Figure 6.
Duration of contractile ring coalescence is longer in formin chimera cells. (A) Confocal time-lapse micrographs of representative cells from indicated strains during coalescence of the contractile ring. For comparison, time 0 is marked by the first appearance of Rlc1-tdTomato fluorescence at the midzone. Time is indicated in minutes. Scale bars, 5 μm. Also, see Video S3. (B) Duration of contractile ring coalescence from onset of cytokinesis node and spot movement. 10 cells were measured for each strain. Two-tailed t-tests for data sets with unequal variance yielded p-values ∗p = 6.09 × 10−3 and ∗∗p = 6.13 × 10−6.