Table 1.
In vitro actin assembly properties of formins used to generate formin chimeras
| Formin | Nucleation efficiency | Elongation rate (subunits s−1μM−1) | Dissociation rate (s−1) |
|---|---|---|---|
| fission yeast Cdc12 | one filament per two to three dimers (20,21) | 10–12 (19, 20, 21) | 4.7–7.0 × 10−5 (20,21) |
| fission yeast For3 | one filament per 170 dimers (20) | 10 (20) | 3.6 × 10−5 (20) |
| budding yeast Bni1 | one filament per ~20 dimers (4,22) | 20–25 (19,22) | 8 × 10−4 (4) |
| mouse Dia2 | one filament per ~4–6a dimers | 12 (19) | 1.3 × 10−4 (19) |
| worm CYK-1 | one filament per 25 dimers (21) | ~60 (21,23) | 3.9 × 10−3 (21) |
| fission yeast Fus1 | one filament per two dimers (20) | 5 (20) | 6.5 × 10−4 (20) |
Formin nucleation efficiency (filaments per dimer), barbed-end elongation rate (in the presence of profilin), and dissociation rate from the barbed end were determined previously with in vitro biochemical assays.
a
Data is as shown in Fig. S4C.