Figure 4.

Myopic cells enhance chromatin condensation in the nucleus that is restored to the level of healthy corneal cells after recovery to the healthy state. (a) Myopic cells exhibit higher level of chromatin condensation than control cells. The CCP of both myopic and control corneal cells was measured by imaging processing to represent the level of chromatin condensation. Representative results of three independent experiments are shown. ^∗p < 0.05. (b) Representative images of chromatin condensation in myopic and control cells. The nuclei of both myopic corneal cells and control cells were stained with DAPI (left panel). The visible edges within the nucleus were identified by CCP image processing (middle panel) and the condensed nuclear area was filled with pseudocolor (right panel). (c) Recovered cells exhibit similar level of chromatin condensation to control cells. Representative results of three independent experiments are shown. NS, no significance. (d) Representative images of chromatin condensation in recovered and control cells. The number of cell samples was shown in the figures. To see this figure in color, go online.