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Biochemical assays. Mass-spectrometry results of reaction products (5 min at 23°C) using tetra-ubiquitin (A) and diubiquitin (B) isomers are shown. Substrates are listed above and enzyme variants used on the left. Positions of substrates and products are shown below. C) Shown are substrate-dependent initial rates determined using the fluorescence-based Ub-AMC assay, with Michaelis-Menten kinetic parameters listed in Table II.
