TABLE 2.
A Gal4DBD-Sko1 fusion protein confers osmotic regulation to the heterologous GAL1 promotera
| Strain | GAL4DBD-SKO1 | β-Galactosidase sp act (nmol/min/mg)
|
Induction factor | |
|---|---|---|---|---|
| YPD | 0.3 M NaCl | |||
| Wild type | − | 1.08 ± 0.19 | 1.40 ± 0.10 | 1.3 |
| + | 0.24 ± 0.06 | 2.39 ± 0.63 | 10 | |
| ssn6 | − | 1.51 ± 0.20 | 1.26 ± 0.11 | 0.8 |
| + | 1.36 ± 0.16 | 2.74 ± 0.32 | 2.0 | |
| tup1 | − | 3.51 ± 0.49 | 2.93 ± 0.39 | 0.8 |
| + | 4.86 ± 0.58 | 9.25 ± 0.86 | 1.9 | |
a
The GAL4DBD-SKO1 fusion plasmid pMP235 was expressed in wild-type (SFY526) and in Δssn6 (MAP34) and Δtup1 (MAP35) mutant strains. Expression of the integrated GAL1-lacZ reporter was assayed after growth in YPD and YPD–0.3 M NaCl. Strains without the GAL4-SKO1 fusion contained the empty vector pGBT9.