TABLE 3.
ENA1 expression is modulated by multiple repressorsa
| Strain | β-Galactosidase sp act (nmol/min/mg of protein)
|
Derepression factor
|
|||||
|---|---|---|---|---|---|---|---|
| YPD | YPGal | YPD–0.8 M NaCl | YPD–0.8 M KCl | Galactose | 0.8 M NaCl | 0.8 M KCl | |
| Wild type | 6.3 ± 0.6 | 40.9 ± 1.7 | 81.6 ± 4.1 | 32.0 ± 2.8 | 6.5 | 13.0 | 5.1 |
| Δmig1 | 15.4 ± 0.9 | 64.1 ± 7.6 | 130.7 ± 17.1 | ND | 4.2 | 8.5 | ND |
| Δmig2 | 19.8 ± 1.8 | 93.2 ± 10.5 | 192.4 ± 21.5 | ND | 4.7 | 9.7 | ND |
| Δmig1 Δmig2 | 64.8 ± 0.9 | 138.6 ± 13.9 | 287.5 ± 28.9 | ND | 2.1 | 4.4 | ND |
| Δsko1 | 30.5 ± 3.3 | 155.4 ± 17.6 | 126.3 ± 19.3 | 36.9 ± 3.2 | 5.1 | 4.1 | 1.2 |
| Δsko1 Δmig1 Δmig2 | 101.3 ± 11.3 | 185.9 ± 19.8 | 364.5 ± 42.8 | ND | 1.8 | 3.6 | ND |
| Δssn6 | 316.7 ± 27.8 | 399.0 ± 46.1 | 423.7 ± 45.9 | ND | 1.2 | 1.3 | ND |
a
Transcriptional regulation of ENA1 was monitored by using an integrative ENA1-lacZ fusion (pFR70i) under repressed (YPD) and derepressed (YPGal, YPD–0.8 M NaCl, or YPD–0.8 M KCl) growth conditions. The strains used were W303-1A, MAP12, MAP21, MAP24, MAP19, MAP28, and MAP6 (see Table 1 for genotypes). ND, not determined.