Figure EV5. Titration of phosphorylated Bub1^CD1 peptide into Mad1^CTD .

1. ¹H,¹⁵N‐2D HSQC showing ¹⁵N‐labelled Mad1^CTD with an increasing concentration of phosphorylated Bub1^CD1 peptide. In molar ratios of Mad1^CTD dimer to Bub1^CD1 peptides were added at 1:0.5 (red), 1:1 (yellow), 1:2 (blue) and 1:4 (green) ratios.
2. Relative peak intensities of Mad1^CTD upon titration of phosphorylated Bub1^CD1 peptides. The bar charts follow a similar colour scheme as the spectra in A, with molar ratios of Mad1^CTD dimer to Bub1^CD1 at 1:0.5 (red), 1:1 (yellow), 1:2 (blue) and 1:4 (green) ratios. Peak intensities were normalized to that of the C‐terminal residue Ala718.

Source data are available online for this figure.