Table 1.
Treatment (Combination of Harvest Maturity and Temperature Treatment) | ANOVA (Harvest Maturity × Treatment) |
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Compounds | RI Z | Rn y | Rh | Rc | Ln | Lh | Lc | Pn | Ph | Pc | Tn | Th | Tc | Bn | Bh | Bc | Mn | Mh | Mc | ||
ALDEHYDES | |||||||||||||||||||||
1 | Methacrolein | 584 | 0.020 d x | 0.027 d | 0.100 ab | 0.027 d | 0.020 d | 0.100 a | 0.033 d | 0.053 cd | 0.087 bc | 0.091 bc | 0.085 bc | 0.116 ab | 0.091 bc | 0.093 bc | 0.103 ab | 0.117 ab | 0.082 bc | 0.117 ab | ** |
2 | Butanal | 590 | 0.009 g | 0.009 g | 0.024 ab | 0.008 g | 0.009 g | 0.019 b–d | 0.015 c–e | 0.016 c–e | 0.02 bc | 0.018 cd | 0.018 cd | 0.015 d–f | 0.016 c–e | 0.017 c–e | 0.019 b–d | 0.01 fg | 0.012 e–g | 0.026 a | ** |
3 | 3-Methylbutanal # | 638 | 0.113 hi | 0.198 fg | 0.091 i | 0.138 g–i | 0.164 gh | 0.176 gh | 0.295 de | 0.329 de | 0.18 gh | 0.408 bc | 0.558 a | 0.203 fg | 0.602 a | 0.625 a | 0.267 ef | 0.460 b | 0.619 a | 0.344 cd | ** |
4 | 2-Methylbutanal # | 646 | 0.08 gh | 0.146 fg | 0.152 fg | 0.038 h | 0.106 gh | 0.264 de | 0.391 c | 0.447 bc | 0.305 d | 0.483 ab | 0.539 a | 0.212 ef | 0.534 a | 0.495 ab | 0.304 d | 0.395 c | 0.421 bc | 0.405 c | ** |
5 | 2-Methyl-2-butenal | 719 | 0.028 de | 0.041 d | - w | 0.010 e | 0.036 d | - | 0.076 c | 0.089 bc | - | 0.104 b | 0.089 bc | - | 0.125 a | 0.130 a | - | 0.084 bc | 0.099 b | - | ** |
6 | Tiglic aldehyde | 1101 | 0.111 e | 0.164 e | - | 0.041 f | 0.143 e | - | 0.303 d | 0.357 cd | - | 0.414 b | 0.357 cd | - | 0.499 a | 0.520 a | - | 0.336 d | 0.394 bc | - | ** |
7 | trans-Penten-1-al | 1131 | 0.136 c | 0.107 cd | 0.023 f | 0.210 b | 0.140 c | 0.092 d | 0.137 c | 0.081 de | 0.084 de | 0.221 b | 0.211 b | 0.230 b | 0.242 b | 0.098 cd | 0.079 de | 0.312 a | 0.100 cd | 0.042 ef | ** |
8 | cis-3-Hexenal # | 771 | 23.833 bc | 12.681 f | 4.485 g | 28.839 b | 16.613 d–f | 11.931 f | 27.966 b | 16.269 d–f | 13.428 ef | 28.782 b | 21.748 cd | 26.528 bc | 26.017 bc | 17.851 de | 17.993 de | 37.889 a | 18.179 de | 11.427 f | ** |
9 | Hexanal # | 774 | 1.113 d | 0.784 ef | 0.892 d–f | 1.045 de | 0.813 ef | 1.075 de | 1.711 bc | 1.017 de | 1.013 de | 1.973 ab | 1.553 c | 1.079 de | 1.896 ab | 0.965 d–f | 0.774 ef | 2.045 a | 0.964 d–f | 0.670 f | ** |
10 | trans-2-Hexenal # | 828 | 2.056 de | 1.339 gh | 0.503 i | 2.446 cd | 1.588 e–h | 1.540 e–h | 3.340 b | 1.853 e–g | 1.766 e–g | 3.535 b | 2.691 c | 2.625 c | 3.852 b | 1.999 d–f | 1.450 f–h | 4.413 a | 1.936 d–f | 1.142 h | ** |
11 | Heptanal | 875 | 0.008 c | 0.009 c | 0.011 c | 0.007 cd | 0.003 d | 0.015 bc | 0.014 bc | 0.010 c | 0.010 c | 0.017 b | 0.014 bc | 0.014 bc | 0.016 b | 0.008 c | 0.010 c | 0.025 a | 0.010 c | 0.009 c | ** |
12 | trans, trans-2, 4-Hexadienal | 887 | - | - | - | - | - | - | - | - | - | - | 0.613 a | - | - | - | - | - | - | - | ** |
13 | Benzaldehyde | 945 | 0.010 bc | 0.006 de | 0.002 ef | 0.010 bc | 0.003 d–f | 0.007 cd | 0.011 b | 0.004 d–f | 0.002 ef | 0.015 a | 0.01 bc | 0.006 de | 0.011 b | 0.001 f | 0.002 ef | 0.018 a | 0.003 d–f | 0.002 ef | ** |
14 | Octanal | 969 | 0.001 b | 0.002 b | 0.001 b | 0.001 b | - | 0.002 b | - | 0.001 b | 0.004 a | - | 0.001 b | 0.001 b | - | - | 0.001 b | 0.001 b | - | - | ** |
15 | 2-phenylacetaldehyde | 1049 | 0.366 i | 0.375 i | 0.887 c–h | 0.444 g–i | 0.296 i | 0.93 b–f | 1.334 a–c | 0.674 e–i | 0.499 f–i | 1.125 b–e | 1.31 ab | 1.148 b–d | 0.859 c–h | 0.504 f–i | 0.865 c–g | 1.742 a | 0.844 d–h | 0.437 hi | ** |
16 | 2-Octenal | 1074 | 0.032 ab | 0.038 a | - | 0.037 a | 0.003 c | - | 0.034 a | - | - | - | 0.014 bc | - | - | - | - | 0.034 a | - | - | ** |
17 | Nonanal | 1059 | - | - | - | 0.001 a | - | - | - | - | - | - | - | - | - | - | - | - | - | - | ** |
18 | Neral # | 1180 | 0.006 | - | 0.004 | - | 0.005 | - | 0.009 | 0.005 | - | - | - | - | - | - | - | 0.010 | - | - | NS |
HYDROCARBONS | |||||||||||||||||||||
1 | α-Pinene | 910 | - | 0.001 | - | - | - | - | - | - | 0.001 | - | - | 0.001 | - | - | 0.001 | - | - | 0.001 | NS |
2 | Cymene | 994 | - | - | - | - | - | - | - | - | - | - | - | 0.001 | - | - | 0.001 | - | - | 0.001 | NS |
3 | Limonene | 998 | 0.024 e–g | 0.005 g | 0.068 d | 0.009 fg | 0.012 fg | 0.077 d | 0.007 fg | 0.011 fg | 0.06 de | 0.050 d–f | 0.116 c | 0.342 a | 0.062 de | 0.044 d–g | 0.330 a | 0.084 cd | 0.123 c | 0.281 b | ** |
4 | Terpinolene | 1048 | - | - | - | - | - | - | - | - | - | - | - | 0.001 a | - | - | 0.001 a | - | - | - | ** |
ALCOHOLS | |||||||||||||||||||||
1 | 2-Methylpropanol | 612 | 0.006 f | 0.018 de | - | - | 0.014 ef | 0.019 c–e | 0.017 de | 0.022 c–e | 0.067 a | 0.033 b–d | 0.020 c–e | 0.009 ef | 0.035 bc | 0.048 b | 0.014 ef | 0.005 ef | 0.062 a | 0.076 a | ** |
2 | 1-Penten-3-ol | 1157 | 0.055 d | 0.031 e | 0.028 e | 0.085 b | 0.067 b–d | 0.067 b–d | 0.034 e | 0.030 e | 0.054 d | 0.085 b | 0.073 b–d | 0.083 bc | 0.086 b | 0.057 d | - | 0.109 a | 0.065 cd | - | ** |
3 | 3-Methylbutanol # | 707 | 0.365 de | 0.656 cd | 0.068 g | 0.329 d–f | 0.379 de | 0.222 ef | 0.315 d–f | 0.443 d | 0.183 fg | 0.604 d | 0.767 c | 0.296 d–f | 0.927 b | 1.189 a | 0.368 de | 0.770 c | 1.106 a | 0.350 de | ** |
4 | 2-Methylbutanol # | 711 | 0.190 g–i | 0.270 gh | 0.067 i | 0.144 hi | 0.244 gh | 0.417 ef | 0.292 fg | 0.424 ef | 0.613 bc | 0.508 c–e | 0.489 c–e | 0.313 fg | 0.599 b–d | 0.703 ab | 0.567 b–d | 0.464 de | 0.606 b–d | 0.761 a | ** |
5 | 1-Pentanol | 775 | 0.066 b | 0.091 a | 0.021 ef | 0.049 bc | 0.051 bc | 0.026 d–f | 0.009 fg | 0.044 cd | 0.025 def | 0.052 bc | 0.042 c–e | 0.026 d–f | 0.057 bc | 0.041 c–e | 0.016 f | 0.052 bc | 0.022 ef | - | ** |
6 | 4-Methylpentanol | 809 | - | 0.003 b | 0.005 b | 0.002 b | 0.003 b | - | - | - | - | - | - | - | 0.009 b | 0.039 a | - | 0.009 b | 0.031 a | - | ** |
7 | 3-Methylpentanol | 817 | 0.015 d–f | 0.013 f | 0.011 f | 0.015 d–f | 0.013 ef | 0.023 bc | 0.014 ef | 0.015 d–f | 0.023 bc | 0.018 c–e | 0.020 b–d | 0.013 ef | 0.030 a | 0.023 bc | 0.013 ef | 0.025 ab | 0.019 c–e | 0.019 c–e | ** |
KETONES | |||||||||||||||||||||
1 | Acetone | 533 | 0.423 f | 0.453 f | 3.090 ab | 2.004 d | 1.363 e | 2.939 a–c | 1.096 e | 0.727 ef | 2.335 cd | 1.998 d | 1.988 d | 3.106 a | 2.569 a–d | 2.389 cd | 2.45 b–d | 2.447 a–d | 1.285 e | 2.171 d | ** |
2 | 2-Butanone | 591 | 0.002 b | 0.013 a | - | - | - | 0.003 b | - | - | - | - | - | - | - | - | - | - | - | - | ** |
3 | 1-Penten-3-one # | 665 | 0.197 b–d | 0.124 ef | 0.071 f | 0.253 ab | 0.232 a–c | 0.172 c–e | 0.139 de | 0.147 de | 0.129 ef | 0.244 ab | 0.203 a–d | 0.230 a–c | 0.265 a | 0.175 c–e | 0.008 g | 0.255 ab | 0.146 de | 0.005 g | ** |
4 | 6-Methyl-5-hepten-2-one # | 950 | 0.084 i | 0.078 i | 0.279 a–e | 0.107 hi | 0.082 i | 0.329 a | 0.204 e–g | 0.161 gh | 0.248 c–f | 0.254 b–f | 0.205 e–g | 0.304 a–c | 0.216 d–g | 0.197 fg | 0.287 a–d | 0.317 ab | 0.190 fg | 0.247 c–f | ** |
5 | Geranyl acetone # | 1367 | 0.345 ef | 0.084 h | 0.295 e–g | 0.233 fg | 0.203 g | 0.332 ef | 0.553 c | 0.201 g | 0.307 e–g | 0.676 ab | 0.401 de | 0.491 cd | 0.753 a | 0.271 fg | 0.408 de | 0.592 bc | 0.232 fg | 0.227 fg | ** |
OXYGEN-CONTAINING HETEROCYCLIC COMPOUNDS | |||||||||||||||||||||
1 | 2-Methylfuran | 602 | 0.006 f | 0.011 ef | 0.062 a | 0.005 f | 0.009 ef | 0.064 a | 0.010 ef | 0.014 d–f | 0.030 b | 0.013 d–f | 0.016 de | 0.024 bc | 0.008 ef | 0.011 ef | 0.024 bc | 0.014 d–f | 0.007 f | 0.020 cd | ** |
2 | 2-Ethyl furan | 676 | 0.014 b–d | 0.010 d–f | 0.010 c–f | 0.016 b | 0.011 b–f | 0.015 b–d | 0.014 b–e | 0.008 f | 0.010 c–f | 0.023 a | 0.021 a | 0.015 bc | 0.023 a | 0.014 b–e | 0.009 ef | 0.027 a | 0.014 b–e | 0.006 f | ** |
3 | 2-Pentyl-furan | 996 | 0.054 d–f | 0.056 d–f | 0.267 b | 0.057 d–f | 0.034 ef | 0.256 b | 0.088 d | 0.080 d | 0.074 de | 0.064 de | 0.045 d–f | 0.298 b | 0.145 c | 0.016 f | 0.395 a | 0.133 c | 0.080 d | 0.401 a | ** |
ESTERS | |||||||||||||||||||||
1 | Butyl acetate | 774 | 0.001 c | 0.003 bc | 0.004 ab | 0.002 c | 0.002 c | 0.003 c | 0.005 a | 0.004 ab | 0.001 c | 0.003 bc | 0.005 ab | 0.001 a | 0.003 bc | 0.003 bc | - | 0.003 bc | 0.004 ab | 0.001 c | ** |
2 | 2-Methylbutyl acetate | 847 | - | - | - | - | - | - | - | 0.001 a | - | - | 0.001 a | - | - | 0.001 a | - | 0.001 a | 0.001 a | - | ** |
3 | Methyl salicylate # | 1156 | 0.003 | 0.009 | - | 0.011 | 0.013 | - | 0.004 | 0.001 c | 0.002 | 0.005 | 0.003 | - | - | - | - | - | - | - | NS |
SULFUR- AND NITROGEN-CONTAINING HETEROCYCLIC COMPOUNDS | |||||||||||||||||||||
1 | 2-Isobutylthiazole # | 1002 | 0.002 de | 0.003 b–e | - | 0.001 e | 0.001 e | 0.002 c–e | 0.004 a–c | 0.004 a–c | 0.003 b–e | 0.004 a–c | 0.006 a | 0.005 a | 0.004 a–c | 0.002 de | 0.004 a–c | 0.003 b–e | 0.001 e | 0.003 b–e | ** |
SULFUR COMPOUNDS | |||||||||||||||||||||
1 | Disulfide, dimethyl | 752 | 0.001 b | 0.001 b | 0.001 b | 0.001 b | 0.001 b | 0.001 b | 0.001 b | - | - | - | - | 0.004 a | - | - | - | - | - | - | ** |
TOTAL VOLATILES | 29.78 ef | 17.86 j | 11.52 k | 36.63 cd | 22.68 hi | 21.12 ij | 38.48 b–d | 23.54 ghi | 21.56 ij | 41.82 b | 34.23 de | 37.74 b–d | 40.55 bc | 28.53 fg | 26.76 f–h | 51.44 a | 29.42 f–g | 19.19 ij | ** |
z: RI = retention index on a HP-5 column (Agilent). y: Abbreviations of combination of harvest maturity (R—red; L—light red; P—pink; T—turning; B—breaker; and M—mature green) and temperature treatment (h—heating; c—chilling; and n—non-treated control). x: Means followed by different superscripts in the same row indicate significant differences using the Duncan’s multiple range test (p < 0.05). w: Not detectable. ** p < 0.001. NS: not significant. # 13 important volatiles which are deemed key tomato volatiles [11,27,28,29] and were selected for PCA analysis.