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. 2021 Aug 16;10(8):2100. doi: 10.3390/cells10082100

Figure 4.

Figure 4

Immunocytochemistry of the third passage (subculture) (A) Immunocytochemistry using an anti-Vimentin Clone V9 antibody, of a 20% confluent culture, showing typical spindle cell morphology, 40×; (B) Immunocytochemistry using an anti-Cytokeratin AE1/AE3 antibody, of a 20% confluent culture, showing typical spindle cell morphology, 40×. Cells were fixated at lower confluence to allow a better observation of the cell morphology. (C) Immunocytochemistry using an anti-Vimentin Clone V9 antibody, of a 70% confluent culture, 100× (D) Immunocytochemistry using an anti-Cytokeratin AE1/AE3 antibody, of a 70% confluent culture, 100×.