Table 1.
Study of mitochondrial oxygen consumption.
| Conditions | Control | CORT | MPP+ | MPP+ + CORT |
|---|---|---|---|---|
| Basal respiration | 20.9 ± 1.8 | 21.8 ± 1.1 | 12.8 ± 1.3 * | 9.1 ± 2.1 * |
| ATP Production | 16.0 ± 1.7 | 17.9 ± 0.9 | 10.1 ± 1.5 * | 8.9 ± 2.1 * |
| Proton leak | 5.4 ± 0.5 | 4.6 ± 1.1 | 3.6 ± 0.9 | 7.4 ± 1.6 * |
| Spare respiratory capacity | 20.2 ± 1.2 | 8.5 ± 1.4 * | 10.4 ± 0.9 * | 9.9 ± 3.1* |
| Maximal respiration | 41.1 ± 1.2 | 30.2 ± 1.8 * | 23.1 ± 0.6 *,& | 20.5 ± 1.9 *,& |
Data represent the oxygen consumption rate (OCR) in SN4741 neuronal cells assessed in different conditions: basal respiration, after 90 min of incubation with corticosterone (CORT), MPP+ and their combination (CORT + MPP+). ATP production, measured after addition of oligomycin. Proton leak, considered as the residual O2 consumption after oligomycin addition. Spare respiratory capacity, as the difference between maximal respiration and basal respiration. OCR are expressed as pmol/min mg of protein. Data were combined from three independent experiments and presented as mean ± SEM. * p < 0.05 compared to control cells; & p < 0.05 compared with CORT incubated cells.