Figure 4.

Overexpression of NtΔCCC1 improves iron extraction from media. (A) Yeast cells described in Figure 2 were inoculated at an OD_600nm of 0.2 in SC-Ura with 500 μM FAS in a 96-well microplate reader and duplication times were calculated from the resulting growth curves as described in Materials and Methods. (B–D) Yeast cells described in Figure 2 were cultivated in SC-Ura + 500 μM FAS for 6 h, and OD_600nm (B), μg Fe/g dry weight (DW) (C), and μg Fe/L of medium (D) were determined. Three independent biological replicates were performed. The average and standard deviation were determined. Different letters above the bars represent statistically significant differences (p-value < 0.05).