Table 1.

Composition of simulated digestive fluids used for the in vitro digestion.

			SSF pH 7.0	SGF pH 3.0	SIF pH 7.0
	Conc. in Stock		Conc. in SSF	Conc. In SGF	Conc. in SIF
	g/L	mol/L	mmol/L	mmol/L	mmol/L
KCl	37.3	0.5	15.1	6.9	6.8
KH2PO4	68	0.5	3.7	0.9	0.8
NaHCO3	84	1	13.6	25	85
NaCl	117	2	—	47.2	38.4
MgCl2(H2O)6	30.5	0.15	0.15	0.1	0.33
(NH4)2CO3	48	0.5	0.06	0.5	—
For pH adjust
NaOH		1.0
HCl		6.0	1.1	15.6	8.4
CaCl2 (H2O)2	44.1	0.3	1.5 (0.075 *)	0.15 (0.075 *)	0.6 (0.3 *)
Salivary amylase			75 U/mL	—	—
Pepsin			—	2000 U/mL	—
Pancreatin			—	—	100 U/mL **
Bile			—	—	10 mM

* Ca²⁺ concentration in the final digestion mixture. ** Sufficient pancreatin to provide 100 U/mL of trypsin (TAME Units).