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. 2021 Jul 30;10(8):1940. doi: 10.3390/cells10081940

Figure 3.

Figure 3

Exogenously added fatty acids are not accumulated in the peroxisomes or mitochondria. C. reinhardtii UVM4 expressing CIS2-CFP (cyan) was observed with confocal laser scanning microscopy after adding 20 µM FL-C16 (yellow), and 10 µM MitoTracker (red). Thirty optical sections of a 20 µm z-stack for each signal are projected into one image with their maximum intensity, respectively. Independent fluorescence channels are shown for CIS2-CFP, FL-C16, and MitoTracker as well as an overlay of these signals (C/F/MT). Chlorophyll fluorescence and transmission image are also shown. CIS2 is the citrate synthase, a key enzyme characterized to localize in algal peroxisomes where it participates in the glyoxylate cycle. Neither the signals of CIS2-CFP or MitoTracker co-localize with FL-C16. Chlorophyll autofluorescence is shown in green.