FIG. 8.

Meiosis-specific DSBs at the THR4 locus. The physical map of the THR4 region of chromosome III indicating the EcoRI sites, location of the probe, and approximate site of the DSB is shown at the top of the figure. A time course (in hours) of meiosis of wild-type, rad50S, and mre11-H125N strains is shown at the bottom. DNA samples from each time point were digested with EcoRI. The positions of the parental and DSB fragments are indicated to the right of the autoradiogram, and the migration positions of molecular size markers (in kilobases) are given to the left.