TABLE 1.
Yeast strains used in this study
| Strain | Relevant genotypea or description | Source |
|---|---|---|
| SK-1 derivatives | ||
| AMP268 | MATα his4-G | A. Mitchell |
| AMP464 | MATa his4-N | A. Mitchell |
| LSY675 | Diploid of AMP268 × AMP464 | This study |
| LSY674 | MATa his4-N mre11::LEU2 | This study |
| LSY649 | MATα his4-G mre11::LEU2 | This study |
| LSY660 | Diploid of LSY674 × LSY649 | This study |
| W303 derivatives | ||
| W303-1A | MATa | R. Rothstein |
| W303-1B | MATα | R. Rothstein |
| W303 | Diploid of W303-1A × W303-1B | This study |
| YBL2 | MATα rad27::TRP1 | 59 |
| YAR70 | MATα spo13::hisG-URA3-hisG | A. Rattray |
| HKY677-11C | MATamei4::ADE2 | H. Klein |
| MAY1-21A | MATα leu2-K::ADE2-URA3::leu2-R rad50S::URA3 | H. Klein |
| MAY1-21D | MATa leu2-K rad50S::URA3 | H. Klein |
| R877 | MATα hdf1::LEU2 | R. Rothstein |
| LSY525-1C | MATa mei4::ADE2 spo13::hisG-URA3-hisG | This study |
| LSY525-3A | MATα mei4::ADE2 spo13::hisG-URA3-hisG | This study |
| LSY568 | MATamre11::LEU2 | This study |
| LSY569 | MATα mre11::LEU2 | This study |
| LSY730 | Diploid of LSY568 × LSY569 | This study |
| LSY716 | MATamre11-H125N::URA3::mre11-D56N | This study |
| LSY716A | MATa mre11-H125N | This study |
| LSY726 | MATα mre11-H125N::URA3::mre11-D56N | This study |
| LSY726A | MATα mre11-H125N | This study |
| LSY729 | Diploid of LSY716 × LSY726 | This study |
| LSY731 | MATamei4::ADE2 | This study |
| MATα mei4::ADE2 | ||
| LSY732 | MATaspo13::hisG-URA3-hisG | This study |
| MATα spo13::hisG-URA3-hisG | ||
| LSY733 | MATamre11::LEU2spo13::hisG-URA3-hisG | This study |
| MATα mre11::LEU2 spo13::hisG-URA3-hisG | ||
| LSY734 | MATamre11-H125Nspo13::hisG-URA3-hisG | This study |
| MATα mre11-H125N spo13::hisG-URA3-hisG | ||
| LSY735 | MATamre11-H125Nspo13::hisG-URA3-hisGmei4::ADE2 | This study |
| MATα mre11-H125N spo13::hisG-URA3-hisG mei4::ADE2 | ||
| LSY738 | MATaspo13::hisG-URA3-hisGmei4::ADE2 | This study |
| MATα spo13::hisG-URA3-hisG mei4::ADE2 | ||
| LSY741 | MATarad50S::URA3leu2-K | This study |
| MATα rad50S::URA3 leu2-K::ADE2-URA3::leu2-R | ||
| LSY736 | MATa hdf1::LEU2 mre11::LEU2 | This study |
| LSY737 | MATa hdf1::LEU2 mre11-H125N | This study |
| Other | ||
| YDS371 | MATa leu2 trp1 ura3-52 prb1-1122 pep4-3 prc1-407 GAL+ | D. Shore |
a
SK-1 derivatives have the genotype ura3 leu2::hisG trp1::hisG lys2 ho::LYS2 and W303 derivatives have the genotype leu2-3,112 trp1-1 can1-100 ura3-1 ade2-1 his3-11,15; differences from these genotypes are listed in the table.