Figure 4.

Electrophysiological assessment of pancreatic β-cell Ca2+ currents in response to s-cal14.2b. Voltage-gated calcium channel currents (Cav1.2/1.3) were recorded in response to s-cal14.2b using the patch-clamp technique in whole-cell configuration. (A) Time-course of Ca²⁺ current in β-cells following 100 µg/mL s-cal14.2b microperfusion. The gray area indicates application time of s-cal14.2b or 100 µM Cd²⁺. (B) Box plot shows aggregated data of Ca²⁺ current before (control) and after s-cal14.2b and Cd²⁺ blockade. (C) Average increase in Ca²⁺ current amplitude with s-cal14.2b and Cd²⁺ blockade. (D) Summary of time-constant (τ) of the increase and recovery of Ca²⁺ current amplitude with s-cal14.2b-treatment. Thus, s-cal14.2b significantly augmented calcium influx through Ca_V 1.2/1.3 channels. Data are presented as mean ± SE (n = 5). Statistical significance compared to control is indicated by * where p < 0.05.