Figure 5.

Proteasome activity in piglets randomized to hypoxia-ischemia (HI) or sham procedure, and oleuropein (OLE) or vehicle. All groups except for the naïve unanesthetized control group received hypothermia. (A,B) Example assay reaction data in somatosensory cortex and white matter with 100 µg protein in the fluorescence proteasome activity assay. (C,D) The slope of proteasome activity over assay reaction time increased in the cortex and white matter with greater assay protein input among HI OLE (cortex: p < 0.001; white matter: p < 0.001), HI vehicle (cortex: p = 0.023; white matter: p = 0.013), and naïve piglets (cortex: p = 0.002; white matter: p = 0.007). (E) In the cortex, the starting (t₀) proteasome activity increased with greater protein input in sham OLE (p < 0.001) and naïve piglets (p < 0.001). (F) The white matter’s t₀ proteasome activity increased with higher protein input in sham OLE (p < 0.001), sham vehicle (p = 0.002), and naïve groups (p = 0.021). Data are shown as means with standard errors of the means. * p < 0.05.