Figure 9.

Correlations between immunoreactivity for PA28γ and proteasome 20S subunits α3 and β5 and between PA28γ and proteasome activity in piglets that received left intracerebral adeno-associated virus (AAV). Data from the somatosensory cortex (A–D) and subcortical white matter (E–H) are shown. PA28γ and α3 levels had a negative correlation in the cortex (r = −0.833, p = 0.015, A) but no relationship in white matter (r = 0.595, p = 0.132, E). PA28γ correlated positively with β5 in the cortex (r = 0.738, p = 0.046, B) and white matter (r = 0.810, p = 0.022, F). The PA28γ level also correlated with the slope of white matter proteasome activity over assay reaction time (r = 0.833, p = 0.015, G) and starting t₀ activity (r = 0.857, p = 0.011, H). This relationship did not occur in the cortex (slope: r = 0.143, p = 0.752, C; t₀: r = −0.405, p = 0.327, D). The shapes of the data points indicate the cerebral hemispheric AAV dose: square = 2×10¹⁰–2×10¹¹ genome copies (gc) and triangle = 5×10¹⁰–5×10¹¹ gc. Black: AAV-GFP control, red: AAV-shRNA to PA28γ-GFP, and blue AAV-PA28γ-GFP (enforce). Piglets represented by an open symbol also received the adjuvant (40 ng of F108 and 2.5 µg of polybrene).