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. 2021 Jul 29;10(8):1928. doi: 10.3390/cells10081928

Figure 1.

Figure 1

Expression of P2X7R, PSC markers and calcium signals in mPSCs and hPSCs. (a) RT-PCR results with different pairs of primers for the P2X7R (Table 1). (b) Representative blots of P2X7R (70 kDa) isoform A and the loading control β-actin. (c) Immunofluorescence detection of P2X7R and the PSC markers desmin, vimentin, GFAP and α-SMA. Image of mPSCs stained for desmin also shows less activated/quiescent cells with autofluorescent lipid droplets (arrow). Scale bar is 50 μm in all images. (d) Fura-2 ratio in PSCs after consecutive stimulation with 10 μM BzATP and 100 μM BzATP. The graph shows 5 representative cells out of 10–20 cells recorded per experiment, 6 experiments.