Role of P2X7R in cell proliferation in (a) hPSCs and mPSCs from (c) P2X7R wt and (d) P2X7R P451L mice. Cells were treated with different concentrations of the agonist BzATP (0, 10, 100, 1000 μM) with/without 10 μM inhibitor AZ10606120 (AZ) or A438079 (A43) in 1% FBS. Data are normalized to the control value (CTR). We performed one-sample t-test followed by Bonferroni correction and significance compared to the control is indicated as follows: * p < 0.05, ** p < 0.01, **** p < 0.0001. Comparisons between agonist and agonist + inhibitor have been evaluated with t-test and significance is indicated by # p < 0.05, ### p < 0.001. (b) Number of PSCs isolated from the pancreas of either P2X7R wt or P2X7R P451L mice, p = 0.0059.