Figure 6.

Metformin attenuates high-glucose-induced cell death. By using a luminogenic Caspase-Glo^® 3/7 assay, we evaluated the effects of increasing concentrations of metformin on the apoptosis of human brain microvascular endothelial cells induced by high-glucose (Glu: glucose; Mann: mannitol). The experiments were performed in triplicate. Box plots indicate upper/lower quartiles, the line in the middle of each box is the mean, and the whiskers represent the 5th–95th percentile range of values; *: p < 0.05 vs. Glu 5 mM; #: p < 0.05 vs. Vehicle.