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Flow cytometry analysis of the cell cycle 48 h after treatment in: (A) PANC-1; (B) BxPC-3; (C) MIA Paca-2. Analysis of cyclin E protein levels in: (D) PANC-1; (E) BxPC-3; (F), and MIA Paca-2. Densitometry analysis plots show arbitrary units calculated as cyclin E signal normalized to GADPH signal. Conditions for (A,D): MNP 0.5 mg Fe/mL, GEM 22.5 µM, MNP-GEM 0.5 mg Fe/mL 22.5 µM; (B,C,E,F): MNP 0.1 mg Fe/mL, GEM 4.5 µM, MNP-GEM 0.1 mg Fe/mL 4.5 µM.
