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. 2021 Aug 10;13(16):4025. doi: 10.3390/cancers13164025

Figure 5.

Figure 5

Identification of a negative androgen response element in the hTERT proximal promoter. (A) Overview of proximal hTERT deletion constructs for the reporter assays used. The sections highlighted in red are indicative of androgen-mediated repression, whereas the black line does not suggest an androgen-mediated response. (B–E) Luciferase assays with indicated hTERT promoter spanning the proximal promoter region constructs in similar experimental setup as in Figure 4. e.v.—empty vector lacking hTERT sequences or (E) the cDNA for the receptor (B); ARwt—wild-type AR; AR-T877A—mutant AR, R1881 (1 nM). Numbers above the bars indicate the fold induction by androgen treatment. s—sense; as—antisense orientation of the inserted hTERT fragment. * p ≤ 0.05, ** p ≤ 0.01.