Figure 2.

Glycolysis activity as a function of media composition and oxygen. (A) MCF7, (B) Huh7, (C) LNCaP, and (D) SaOS2 cell lines were cultured at either 5% O₂ or 18% O₂ in either DMEM or Plasmax for at least two weeks prior to commencing the assay. Extra cellular acidification (ECAR, normalised to protein content) was measured using a Seahorse XF96 Flux Analyzer. Data shown are means ± SEM from 3 independent experiments per condition (n = 43 wells per each condition for each cell line). “#” represents differences between 18%O₂ and 5%O₂ in the same media. “$” represents differences between Plasmax and DMEM when oxygen level is the same. “!” represents differences between 5% O₂/Plasmax vs. 18% O₂/DMEM. Statistical significance was determined by ANOVA followed by Tukey’s post-hoc analysis.