Figure 4.

Oxygen concentration and media type influence mitochondrial network status. Mitochondrial mean network size (# of branches per network) was measured using MiNA tool in (A) MCF7, (B) Huh7 (C) LNCaP, and (D) SaOS2 cell lines. Cells were cultured at either 5% O₂ or 18% O₂ in either DMEM or Plasmax for at least two weeks prior to commencing the measurements. Data shown are means ± SEM from 3 independent experiments per condition (n = 35 cells per each condition for each cell line). “#” represents differences between 18% O₂ and 5% O₂ in the same media. “$” represents differences between Plasmax and DMEM when oxygen level is the same. “!” represents differences between 5% O₂/Plasmax vs. 18% O₂/DMEM. Statistical significance was determined by ANOVA followed by Tukey’s post-hoc analysis.