Figure 3.

The electrophysiology experiments of KCNJ5 157-159delITE mutation. HEK293T cells expressing wild-type (WT) or KCNJ5 157-159delITE mutant (ITE) were whole-cell patched under voltage clamp mode with a holding potential of 0 mV. (A) The membrane potential stimulation profile. The membrane potentials were changes to various potentials with a step of 40 mV from −120 mV and the currents were recorded. (B) The representative current traces from the same cells expressing WT (Left) and ITE (Right). The currents were recorded at 10 min after the formation of whole-cell patch (Control), 5 min after the serial addition of roxithromycin (20 μM, Rox) and Ba²⁺ (1 mM, Rox + Ba²⁺). The red dash lines indicate the zero current. (C) The averages current–voltage relationship of cells expressing WT (Left) and ITE mutant (Right). The currents at the last 10-ms interval during the change of membrane potentials were averaged and plotted against the potentials. Data presented were Mean ± SEM; the sample number for WT and ITE were 5 and 6, respectively. * indicated p < 0.05 and ** indicated p < 0.01.