Figure 3.

ZNF768 depletion impairs proliferation of various lung cancer cell lines. (A) A549 cells, (B) NCI-H441 and (C) NCI-H460 cells were transduced with lentivirus expressing shRNA targeting ZNF768. Cells were selected with puromycin and protein were extracted, or cells were plated and counted every two days. Pictures were taken on the last day of counting. Representative pictures are shown. Western blot analyses were performed to confirm ZNF768 depletion. This experiment was performed at least 2 times for each cell types and cell counts were done in triplicates. Significance was determined by Two-way ANOVA (* p < 0.05 versus control).