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. 2021 Jul 29;12(8):1180. doi: 10.3390/genes12081180

Figure 1.

Figure 1

Opsin minigene splicing assay. Top: The OPN1LW minigene is OPN1LW cDNA with introns 2 and 3 inserted. The introns are labeled, and the numbered red rectangles are the exons that were identical in sequence for all minigenes. The gray rectangle is exon 3, which varied in sequence among the minigenes. The effects of different SNP combinations in exon 3 on splicing were measured by first transfecting HEK293 cells with minigenes. mRNA was isolated from HEK293 cells, and the amount of correctly spliced mRNA was quantified. Data in Table 2 are from reference [22], and the percentage of correctly spliced mRNA was measured using capillary gel electrophoresis.