Figure 9.

Azo-xyloglucan assay to measure LPMO activity. NcLPMO9C activity with azo-xyloglucan in the presence or absence of ascorbic acid. Reactions were run at 55 °C for 10 min in a final volume of 0.6 mL and contained 0.06 mg/mL enzyme, 1% (w/v) substrate, 2.5 mM ascorbic acid in a 100 mM sodium acetate buffer pH 4.5. Reactions were stopped by the addition of 1 mL of 96% ethanol. After centrifugation at 1000× g for 10 min to separate the substrate, the released azo-labelled oligos were quantified by measuring absorbance at 590 nm.