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Soft agar assays to assess the tumorigenicity of Pax3-FKHR and the Pax3-FKHR mutants. NIH 3T3 fibroblasts were infected with high-titer retroviral stocks containing pSRα(ΔHindIII)-tk-Neo (A), Pax3-FKHR (B), Un-1 (C), ΔPD-NH2 (D), ΔHD-C (E), ΔHD (F), Δ728 (G), or Δ772 (H) and cloned in 0.3% soft agar. Colonies were visualized and counted 2 to 3 weeks after plating.
