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TRPs expression in GSCs during differentiation. GSCs (day 0) and D-GSCs (day 7 and 14) were fixed, permeabilized, and stained with anti-human TRPA1 and TRPV1 Abs followed by Alexa Fluor-488 and Alexa Fluor-594 secondary Abs, respectively. 40,6-diamidino-2-phenylindole (DAPI) was used to counterstain nuclei. Magnification 60×.
