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. 2021 Aug 10;10(8):2052. doi: 10.3390/cells10082052

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Phenotype characterization of NSCs: (A) Annexin V staining was analyzed by flow cytometry in in GSC#1 and D-GSC#1 at 7 days of differentiation, treated with CPS (50 and 200 μM) or CINN (50 and 100 μM) for 24 h. A representative one out of three experiments have been shown. Percentages represent the positive cells. MFI = mean fluorescence intensity. (B) The cytotoxic effects in GSC#83 and D-GSC#83 at 7 days of differentiation, treated with CPS (50 and 200 μM) or CINN (50 and 100 μM) were determined by PI staining and cytofluorimetric analysis assay. A representative of three experiments has been shown. Percentages represent the positive cells. MFI = mean fluorescence intensity.