Table 2.
TRPA1 and TRPV1 channels mRNA expression in GSCs.
| Phenotype | Cell Lines | TRPA1 mRNA | TRPV1 mRNA | ||
|---|---|---|---|---|---|
| Relative mRNA Expression ± SD | Fold Expression |
Relative mRNA Expression ± SD |
Fold Expression |
||
| NSC | 0.151 ± 0.014 | 1.00 | 0.554 ± 0.023 | 1.00 | |
| PN-like | GSC#1 | 0.961 ± 0.031 | 6.36 | 0.314 ± 0.089 | 0.57 |
| MES-like | GSC#83 | 8.881 ± 0.101 | 58.81 | 1.774 ± 0.051 | 3.20 |
| PN-like | GSC#23C | 0.071 ± 0.003 | 0.47 | 0.163 ± 0.004 | 0.29 |
| PN-like | GSC#28 | 0.292 ± 0.043 | 1.93 | 0.217 ± 0.023 | 0.39 |
| MES-like | GSC#30 | 0.302 ± 0.003 | 2.00 | 0.015 ± 0.003 | 0.03 |
| PN-like | GSC#68 | 0.452 ± 0.013 | 2.99 | 0.543 ± 0.005 | 0.98 |
| PN-like | GSC#70 | 0.881 ± 0.054 | 5.83 | 0.692 ± 0.022 | 1.25 |
mRNA samples extracted from NSCs and GSCs. Data represents relative mRNA levels and fold expression of individual gene in GSCs compared to the NSCs. The expression levels were normalized to the average Ct value of two housekeeping genes (GAPDH and RPLP0) and calculated by the2−ΔΔCt method.