Table 3.
Aml1b and Aml1c splice variants mRNA expression in D-GSCs.
| Phenotype | Cell Lines | Aml1b mRNA | Aml1c mRNA | ||
|---|---|---|---|---|---|
| Relative mRNA Expression ± SD |
Fold Expression |
Relative mRNA Expression ± SD |
Fold Expression |
||
| PN-like | D-GSC#1 | 2.621 ± 0.031 | 2.15 | 2.813 ± 0.089 | 2.26 |
| MES-like | D-GSC#83 | 5.056 ± 2.101 | 2.08 | 10.085 ± 0.651 | 2.18 |
| PN-like | D-GSC#23C | 1.722 ± 0.032 | 2.39 | 1.988 ± 0.021 | 2.38 |
| PN-like | D-GSC#28 | 2.776 ± 0.035 | 3.79 | 3.500 ± 0.023 | 3.23 |
| MES-like | D-GSC#30 | 7.810 ± 0.013 | 4.00 | 0.646 ± 0.004 | 1.99 |
| PN-like | D-GSC#68 | 2.071 ± 0.107 | 1.20 | 0.612 ± 0.005 | 2.85 |
| PN-like | D-GSC#70 | 1.303 ± 0.046 | 1.06 | 0.998 ± 0.013 | 3.08 |
mRNA samples extracted from GSCs and D-GSCs at 14 days of differentiation. Data represents relative mRNA levels and fold expression of individual gene in D-GSCs compared to the respective GSCs (=1). The expression levels were normalized to the average Ct value of two housekeeping genes (GAPDH and RPLP0) and calculated by the 2−ΔΔCt method.