Table 4.
Phenotype | Cell Lines | TRPA1 mRNA | TRPV1 mRNA | ||
---|---|---|---|---|---|
Relative mRNA Expression ± SD |
Fold Expression |
Relative mRNA Expression ± SD |
Fold Expression |
||
PN-like | D-GSC#1 | 4.622 ± 0.031 | 4.81 | 0.187 ± 0.089 | 0.34 |
MES-like | D-GSC#83 | 38.851 ± 0.101 | 4.49 | 1.202 ± 0.051 | 0.68 |
PN-like | D-GSC#23C | 4.994 ± 0.221 | 212.93 | 0.113 ± 0.011 | 0.69 |
PN-like | D-GSC#28 | 0.122 ± 0.001 | 0.42 | 0.131 ± 0.001 | 0.60 |
MES-like | D-GSC#30 | 1.143 ± 0.013 | 3.78 | 0.007 ± 0.003 | 0.47 |
PN-like | D-GSC#68 | 0.292 ± 0.022 | 0.65 | 0.363 ± 0.023 | 0.67 |
PN-like | D-GSC#70 | 0.227 ± 0.011 | 0.26 | 0.233 ± 0.014 | 0.34 |
mRNA samples extracted from GSCs and D-GSCs at 14 days of differentiation. Data represents relative mRNA levels and fold expression of individual genes in D-GSCs compared to the respective GSCs. The expression levels were normalized to the average Ct value of two housekeeping genes (GAPDH and RPLP0) and calculated by the 2−ΔΔCtmethod.