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. 2021 Aug 10;10(8):2052. doi: 10.3390/cells10082052

Table 4.

TRPA1 and TRPV1 channels mRNA expression in D-GSCs.

Phenotype Cell Lines TRPA1 mRNA TRPV1 mRNA
Relative mRNA
Expression ± SD
Fold
Expression
Relative mRNA
Expression ± SD
Fold
Expression
PN-like D-GSC#1 4.622 ± 0.031 4.81 0.187 ± 0.089 0.34
MES-like D-GSC#83 38.851 ± 0.101 4.49 1.202 ± 0.051 0.68
PN-like D-GSC#23C 4.994 ± 0.221 212.93 0.113 ± 0.011 0.69
PN-like D-GSC#28 0.122 ± 0.001 0.42 0.131 ± 0.001 0.60
MES-like D-GSC#30 1.143 ± 0.013 3.78 0.007 ± 0.003 0.47
PN-like D-GSC#68 0.292 ± 0.022 0.65 0.363 ± 0.023 0.67
PN-like D-GSC#70 0.227 ± 0.011 0.26 0.233 ± 0.014 0.34

mRNA samples extracted from GSCs and D-GSCs at 14 days of differentiation. Data represents relative mRNA levels and fold expression of individual genes in D-GSCs compared to the respective GSCs. The expression levels were normalized to the average Ct value of two housekeeping genes (GAPDH and RPLP0) and calculated by the 2−ΔΔCtmethod.