Table 5.
Analysis of stem cell signaling pathway in PN-like GSC#1 during differentiation.
| Upregulated Genes | Fold Expression ± SD | Downregulated Genes | Fold Expression ± SD |
|---|---|---|---|
| ACVR1 | 2.54 ± 0.20 | E2F5 | −2.45 ± 0.12 |
| AMHR2 | 3.81 ± 0.10 | FZD2 | −2.86 ± 0.11 |
| BMPR2 | 2.66 ± 0.32 | LIFR | −2.91 ± 0.01 |
| CDX2 | 2.40 ± 0.15 | NOTCH3 | −2.95 ± 0.15 |
| FZD1 | 2.77 ± 0.03 | NOTCH4 | −2.00 ± 0.29 |
| FZD3 | 2.69 ± 0.10 | SMAD3 | −2.15 ± 0.08 |
| SMAD2 | 2.67 ± 0.10 | STAT3 | −5.06 ± 0.50 |
Stem Cell Signaling Pathway Finder PCR array in mRNA samples extracted from GSC#1 and D-GSC#1 after 7 days of culture. Values represent fold differences of individual gene expression in D-GSC#1 compared to GSC#1. The expression levels were normalized to the average Ct value of two housekeeping genes (GAPDH and RPLP0) and calculated by the ΔΔCt method. Genes, whose expression is, at least, 2.00-fold up/downregulated, are shown.