Table 6.
Analysis of stem cell signaling pathway in MES-like GSC#83 during differentiation.
| Upregulated Genes | Fold Expression ± SD | Downregulated Genes | Fold Expression ± SD |
|---|---|---|---|
| ACVR1 | 2.85 ± 0.10 | FGFR4 | −11.64 ± 0.08 |
| AMHR2 | 2.55 ± 0.30 | FZD1 | −10.26 ± 0.01 |
| BMPR1B | 2.90 ± 0.29 | LRP6 | −4.83 ± 0.11 |
| CDX2 | 2.00 ± 0.05 | LTBP2 | −7.75 ± 0.10 |
| FGFR2 | 6.70 ± 0.30 | NFAT5 | −2.24 ± 0.09 |
| FZD9 | 3.00 ± 0.15 | NOTCH4 | −8.84 ± 0.51 |
| IL6ST | 3.34 ± 0.10 | RGMA | −2.64 ± 0.61 |
| LIFR | 2.40 ± 0.12 | SMAD3 | −3.33 ± 0.31 |
| RBPJL | 2.05 ± 0.13 | TGFBR2 | −6.40 ± 0.72 |
| SMAD9 | 4.70 ± 0.10 |
Stem Cell Signaling Pathway Finder PCR array in mRNA samples extracted from GSC#83 and D-GSC#83 after 7 days of culture. Values represent fold differences of individual gene expression in D-GSC#83 compared to GSC#83. The expression levels were normalized to the average Ct value of two housekeeping genes (GAPDH and RPLP0) and calculated by the 2−ΔΔCt method. Genes, whose expression was, at least, 2.00-fold up/downregulated, are shown.