Figure 2.
Transcriptional effect of metformin on MECP2E1-E2 and BDNF in Daoy cells under the condition of with and without serum starvation. (A) Daoy cells were serum-starved for 24 h prior to drug treatment with 250 and 1000 μM metformin. Samples were collected at 6 and 24 h and gene-specific nascent and steady RNA transcripts were analyzed by RT-PCR for MECP2E1, MECP2E2, and BDNF. CT values were normalized to the housekeeping gene GAPDH and fold change was determined relative to the average control. N = 3 ± SEM. Fold change values were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test, * p < 0.05. (B) Similar to A, but Daoy cells were subjected to the same drug treatment, without prior serum starvation.