Figure 3.
Transcriptional effect of two lots of metformin on MECP2E1-E2 and BDNF in Daoy cells with serum starvation. (A,B) Daoy cells were serum-starved for 24 h prior to drug treatment with 1000 and 2000 μM metformin (Met) with two lots of the drug from the same company. Samples were collected at 6 and 48 h and gene transcripts were analyzed by RT-PCR for MECP2E1, MECP2E2, and BDNF. CT values were normalized to the housekeeping gene GAPDH and fold change was determined relative to the average control. N = 3 ± SEM. Fold change values were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test, * p < 0.05, ** p < 0.01, *** p < 0.001, and **** p < 0.0001.