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. 2021 Aug 22;11(8):1253. doi: 10.3390/biom11081253

Figure 4.

Figure 4

Transcriptional effect of simvastatin on MECP2E1-E2 and BDNF in Daoy cells under the condition with and without serum starvation. (A) Daoy cells were serum-starved for 24 h prior to drug treatment with 2.5 and 5.0 μM simvastatin. Samples were collected at 6 and 24 h and gene transcripts were analyzed by RT-PCR for MECP2E1, MECP2E2, and BDNF. CT values were normalized to the housekeeping gene GAPDH and fold change was determined relative to the average control. N = 3 ± SEM. Fold change values were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test, * p < 0.05, ** p < 0.01, and *** p < 0.001. (B) Similar to A, but Daoy cells were subjected to the same drug treatment, without prior serum starvation.