Figure 5.
Combined transcriptional effects of simvastatin and metformin on MECP2E1-E2 and BDNF in Daoy cells under the condition with and without serum starvation. (A) Daoy cells were serum-starved prior to drug treatment with 2.5 μM simvastatin (Stat), 250 μM metformin (Met), and a combination of 2.5 μM Stat + 250 μM metformin. Samples were collected at 6 and 24 h and gene transcripts (nascent and steady-state) were analyzed by RT-PCR for MECP2E1, MECP2E2, and BDNF. CT values were normalized to the housekeeping gene GAPDH and fold change was determined relative to the average control. N = 3 ± SEM. Fold change values were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test, * p < 0.05, ** p < 0.01, *** p < 0.001. (B) Similar to A, but Daoy cells were subjected to the same drug treatment without prior serum starvation.