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. 2021 Aug 22;11(8):1253. doi: 10.3390/biom11081253

Figure 7.

Figure 7

Combined transcriptional effect of simvastatin and metformin on ribosomal RNA genes with serum starvation. Daoy cells were serum-starved for 24 h prior to drug treatment with 2.5 μM simvastatin (Stat), 250 μM metformin (Met), and a combination of 2.5 μM Stat + 250 μM metformin. Samples were collected at 6 and 24 h and gene transcripts were analyzed by RT-PCR for 45S pre-rRNA, 28S rRNA, and 18S rRNA transcripts. CT values were normalized to the housekeeping gene GAPDH and fold change was determined relative to the average control. N = 3 ± SEM. Fold change values were analyzed by one-way ANOVA followed by Tukey’s multiple comparisons test.