FIG. 1.

Binding between the separated regulatory and catalytic domains of Pak1. L40 was transformed with either pGAD, pGAD-Pak1-Reg, or pGAD-Cdc42^V12,C189S and either pLBD-Cdc42^V12, pLBD-Pak1-Cat, pLBD-Pak1^K415,416R-Cat, pLBD-Ras1, pLBD-Pak1, or pLBD-Pak1-Reg. Transformants were tested for growth on medium lacking histidine (DO-LTH) and assayed for β-galactosidase production. VSG, very slow growth. Values shown are relative levels (means ± standard deviations). DO-LT is the medium lacking leucine and tryptophan. ND, not determined.