FIG. 2.

Regions on Pak1-Reg mediating the interaction with the kinase catalytic domain. Pak1-Reg deletion mutants were made by PCR and fused to GAD. The GAD fusion to the regulatory domain of Byr2 was included as a control (last row). These fusions were assayed for interactions with LBD fused to Cdc42, Pak1-Cat, or Ras1 as a negative control. A plus sign represents a two-hybrid interaction; a minus sign represents no detectable two-hybrid interaction. The positive interactions were all of about similar intensities. The amino acid positions of the peptide sequences expressed as GAD fusions are shown. The conserved region of the Pak1 regulatory domain is shown in gray.