FIG. 3.
Cyclin E2 forms a catalytically active complex with Cdk2. 293T cells were transiently transfected with empty GFP vector (lanes 1 and 2) or a vector expressing GFP-E2 (lanes 3 and 4) or GFP-E2SV (lanes 5 and 6). Where indicated, HA-Cdk2 (lanes 1 to 6) and p27Kip1 (lanes 1, 4 and 6) plasmids were cotransfected. Immunoprecipitations were performed with GFP antibodies followed by either Western blotting or kinase assays. The top four panels represent Western blot analyses, and the bottom panel represents histone H1 kinase assays performed on the GFP immunoprecipitates.