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Example of PCR-HRM curves for the L7 SNP with alleles C/T. Each curve corresponds to one of the three possible genotypes. In purple (―) control curve C/C, in blue (―) control curve (T/T), in green (―) control curve (C/T) and in grey (―) query samples. (a) Normalized melting curves; (b) difference melting curves, using control curve for genotype T/T as reference.
