Table A6.
LOD6 determination in PAPM and L2.
| SNP | Dilution (ng/μL) | Cq ± SD 1 | Positive and Specific Amplification (%) 1 |
|---|---|---|---|
| PAPM | 25 | 24.29 ± 1.19 | 94.44 |
| 20 | 23.66 ± 1.83 | 77.78 | |
| 15 | 24.14 ± 0.94 | 94.44 | |
| 12 | 24.98 ± 3.57 | 77.78 | |
| 8 | 25.39 ± 2.20 | 83.33 | |
| 5 | 26.87 ± 3.72 | 77.78 | |
| 1.25 | 20.53 ± 7.99 | 11.11 | |
| L2 | 25 | 24.60 ± 0.16 | 100.00 |
| 20 | 25.15 ± 0.10 | 94.44 | |
| 15 | 25.59 ± 0.24 | 100.00 | |
| 12 | 25.96 ± 0.31 | 100.00 | |
| 8 | 26.55 ± 0.20 | 100.00 | |
| 5 | 27.95 ± 0.23 | 100.00 | |
| 1.25 | 20.45 ± 7.19 | 50.00 |
1 Average considering 3 days, with 6 replicates by tested dilution point per day.