Table A8.
Robustness. Differences (Δ = X-x), between each of the selected parameters by SNP and extraction method.
| Parameter | SNP PAPM | SNP L2 | ||
|---|---|---|---|---|
| Phenol–Chloroform | CTAB | Phenol–Chloroform | CTAB | |
| Annealing temperature (A-a) | 0.105 | 0.167 | 0.222 | 0.505 |
| 260/230 ratio (B-b) | 5.251 | 0.382 | 5.191 | 0.214 |
| 260/280 ratio (C-c) | 0.828 | 0.848 | 0.311 | 0.067 |
| Annealing time (D-d) | 0.075 | 0.159 | 0.300 | 7.569 |
| Primer concentration (E-e) | 0.432 | 4.807 | 0.331 | 4.654 |
| Reaction volume (F-f) | 0.942 | 0.207 | 0.618 | 0.662 |
| Reagent (G-g) | 0.709 | 0.683 | 1.249 | 0.761 |
| SDi | 2.921 | 0.688 | 2.890 | 0.736 |
| Acceptance criteria (S) | 3.008 | 0.971 | 2.890 | 0.876 |