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. 2021 Aug 7;10(8):2017. doi: 10.3390/cells10082017

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© 2021 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Glucose and lactate concentration of differentiation medium and dialysis medium were measured at every medium change during the differentiation. (a) Glucose concentration of differentiation medium, (b) lactate concentration of differentiation medium, (c) glucose concentration of dialysis medium, and (d) lactate concentration of dialysis medium. Control: without dialysis, medium change 2 times, DC(2): with dialysis, medium change 2 times, DC(1): with dialysis, medium change 1 time, DC(0): with dialysis, without medium change. Data represent mean ± SD of n = 5–6 from two independent experiments.