Table 1.

Simulation conditions

Systema	Gate modeled?	Lipids	[Na+] (mM)	Restraints	Temperature (K)	Time step (fs)
NR	yes	mix	150	no	303	2
NR_1fs	yes	mix	150	no	303	1
NR_POPC	yes	POPC	150	no	303	1
NR_310K	yes	mix	150	no	310.15	1
NR_V219D	yes	mix	150	no	303	2
GR	no	mix	150	gate	310.15	2
GR_POPC	no	POPC	150	gate	310.15	2
GR_120mM	no	mix	120	gate	310.15	2
GR_V219D	no	mix	150	gate	310.15	2

^aNR stands for no restraints; GR stands for gate restraints.